PAR1 (non-phospho), Proteinase-Activated Receptor 1 Antibody

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Validation of the Proteinase-Activated Receptor 1 in transfected HEK293 cells

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Delivery time appr. 5-8 days

Order number: 7TM0347N
Content: 100 µl
Host: Rabbit

The PAR1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of... more

The PAR1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Proteinase-Activated Receptor 1. It can be used to detect total PAR1 receptors in Western blots independent of phosphorylation. The PAR1 antibody can also be used to isolate and enrich PAR1 receptors from cell and tissue lysates.

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Alternative Names Coagulation Factor II Receptor, Protease-Activated... more

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Alternative Names	Coagulation Factor II Receptor, Protease-Activated Receptor 1
IUPHAR Target ID	347
UniProt ID	P25116
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents part of carboxyl-terminal tail of human PAR1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Proteinase-Activated Receptor 1 in transfected HEK293 cells.... more

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Figure 1. Validation of the Proteinase-Activated Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Proteinase-Activated Receptor 1 (PAR1) were lysed and immunoblotted with the anti-PAR1 antibody (7TM0347N) at a dilution of 1:1000. Note, PAR1 forms stable multimers.